PT  - JOURNAL ARTICLE
AU  - LIN, JING-PIN
AU  - LU, HSU-FENG
AU  - LEE, JAU-HONG
AU  - LIN, JAUNG-GUNG
AU  - HSIA, TE-CHUN
AU  - WU, LII-TZU
AU  - CHUNG, JING-GUNG
TI  - (-)-Menthol Inhibits DNA Topoisomerases I, II α and β and Promotes NF-Î B expression in Human Gastric Cancer SNU-5 Cells
DP  - 2005 May 01
TA  - Anticancer Research
PG  - 2069--2074
VI  - 25
IP  - 3B
4099  - http://ar.iiarjournals.org/content/25/3B/2069.short
4100  - http://ar.iiarjournals.org/content/25/3B/2069.full
SO  - Anticancer Res2005 May 01; 25
AB  - It has been reported that (-)-Menthol can inhibit the growth of rat liver epithelial tumor cells and is a potent chemopreventive agent. The purpose of the present experiment was to examine and identify cellular processes leading to cell death which are affected by (-)-Menthol in human gastric SNU-5 cancer cells. Cell death (cytotoxicity) was examined and analyzed by trypan blue stain and flow cytometric methods. It was shown that (-)-Menthol inhibited the proliferation of the cells in a dose- and time-dependent manner, inhibited topoisomerase I, IIα and IIβ, but promoted the levels of NF-Î B gene expression based on the Western blot and polymerase chain reaction (PCR) and cDNA microarray methods. These data suggest that (-)-Menthol may induce cytotoxicity through inhibiting gene expression of topoisomerase I, IIα and IIβ and promoting the gene expression of NF-Î B in SNU-5 cells. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved