RT Journal Article
SR Electronic
T1 miR-3188 Enhances Sensitivity of Breast Cancer Cells to Ionizing Radiation by Down-regulating Rictor
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6169
OP 6176
DO 10.21873/anticanres.15436
VO 41
IS 12
A1 SUNG-EUN HONG
A1 HYEON-OK JIN
A1 SEUNG-MI KIM
A1 SE-KYEONG JANG
A1 CHAN SUB PARK
A1 MIN-KI SEONG
A1 HYUN-AH KIM
A1 WOO CHUL NOH
A1 IN-CHUL PARK
YR 2021
UL http://ar.iiarjournals.org/content/41/12/6169.abstract
AB Background/Aim: Rictor is an adaptor protein essential for mTORC2, which regulates cell growth and survival. The aim of this study was to identify microRNAs (miR) that down-regulate Rictor and investigate their function on breast cancer cell survival. Materials and Methods: Trypan blue assay, MTT assay, polymerase chain reaction analysis, luciferase reporter assay and western blot analysis were carried out in breast cancer cell lines HCC1954, MDA-MB-231, SK-BR-3, and BT474. Results: miR-3188 overexpression suppressed the expression of Rictor and inhibited cell viability in HCC1954 and MDA-MB-231, highly Rictor-expressing breast cancer cells. In addition, miR-3188 overexpression decreased the protein level of p-AKT at Ser473, a substrate of mTORC2. Moreover, miRNA-3188 overexpression sensitized breast cancer cells to ionizing radiation (IR) by down-regulating Rictor and p-AKT. Conclusion: miR-3188 enhances IR sensitivity by affecting the mTORC2/AKT signalling pathway by altering the expression of Rictor, which could be a promising therapeutic strategy for the future treatment of breast cancer.