TY - JOUR T1 - miR-3188 Enhances Sensitivity of Breast Cancer Cells to Ionizing Radiation by Down-regulating Rictor JF - Anticancer Research JO - Anticancer Res SP - 6169 LP - 6176 DO - 10.21873/anticanres.15436 VL - 41 IS - 12 AU - SUNG-EUN HONG AU - HYEON-OK JIN AU - SEUNG-MI KIM AU - SE-KYEONG JANG AU - CHAN SUB PARK AU - MIN-KI SEONG AU - HYUN-AH KIM AU - WOO CHUL NOH AU - IN-CHUL PARK Y1 - 2021/12/01 UR - http://ar.iiarjournals.org/content/41/12/6169.abstract N2 - Background/Aim: Rictor is an adaptor protein essential for mTORC2, which regulates cell growth and survival. The aim of this study was to identify microRNAs (miR) that down-regulate Rictor and investigate their function on breast cancer cell survival. Materials and Methods: Trypan blue assay, MTT assay, polymerase chain reaction analysis, luciferase reporter assay and western blot analysis were carried out in breast cancer cell lines HCC1954, MDA-MB-231, SK-BR-3, and BT474. Results: miR-3188 overexpression suppressed the expression of Rictor and inhibited cell viability in HCC1954 and MDA-MB-231, highly Rictor-expressing breast cancer cells. In addition, miR-3188 overexpression decreased the protein level of p-AKT at Ser473, a substrate of mTORC2. Moreover, miRNA-3188 overexpression sensitized breast cancer cells to ionizing radiation (IR) by down-regulating Rictor and p-AKT. Conclusion: miR-3188 enhances IR sensitivity by affecting the mTORC2/AKT signalling pathway by altering the expression of Rictor, which could be a promising therapeutic strategy for the future treatment of breast cancer. ER -