TY - JOUR T1 - Tumor-specificity and Apoptosis-inducing Activity of Stilbenes and Flavonoids JF - Anticancer Research JO - Anticancer Res SP - 2055 LP - 2063 VL - 25 IS - 3B AU - SHAHEAD ALI CHOWDHURY AU - KAORI KISHINO AU - RIE SATOH AU - KEN HASHIMOTO AU - HIROTAKA KIKUCHI AU - HIROFUMI NISHIKAWA AU - YOSHIAKI SHIRATAKI AU - HIROSHI SAKAGAMI Y1 - 2005/05/01 UR - http://ar.iiarjournals.org/content/25/3B/2055.abstract N2 - A total of eleven stilbenes [1-6] and flavonoids [7-11] were investigated for their tumor-specific cytotoxicity and apoptosis-inducing activity, using four human tumor cell lines (squamous cell carcinoma HSC-2, HSC-3, submandibular gland carcinoma HSG and promyelocytic leukemia HL-60) and three normal human oral cells (gingival fibroblast HGF, pulp cell HPC, periodontal ligament fibroblast HPLF). All of the compounds, especially sophorastilbene A [1], (+)-α-viniferin [2], piceatannol [5], quercetin [9] and isoliquiritigenin [10], showed higher cytotoxicity against the tumor cell lines than normal cells, yielding tumor-specific indices of 3.6, 4.7, >3.5, >3.3 and 4.0, respectively. Among the seven cell lines, HSC-2 and HL-60 cells were the most sensitive to the cytotoxic action of these compounds. Sophorastilbene A [1], piceatannol [5], quercetin [9] and isoliquiritigenin [10] induced internucleosomal DNA fragmentation and activation of caspases -3, -8 and -9 dose-dependently in HL-60 cells. (+)-α-Viniferin [2] showed similar activity, but only at higher concentrations. All the compounds failed to induce DNA fragmentation and activated caspases to much lesser extents in HSC-2 cells. Western blot analysis showed that sophorastilbene A [1], piceatannol [5] and quercetin [9] did not induce any consistent changes in the expression of pro-apoptotic proteins (Bax, Bad) and anti-apoptotic protein (Bcl-2) in HL-60 and HSC-2 cells. An undetectable expression of Bcl-2 protein in control and drug-treated HSC-2 cells may explain the relatively higher sensitivity of this cell line to stilbenes and flavonoids. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -