RT Journal Article
SR Electronic
T1 Impact of Cytogenetic and Molecular Cytogenetic Studies on Hematologic Malignancies
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2979
OP 2983
VO 25
IS 4
A1 A. KOLIALEXI
A1 G.TH. TSANGARIS
A1 S. KITSIOU
A1 E. KANAVAKIS
A1 A. MAVROU
YR 2005
UL http://ar.iiarjournals.org/content/25/4/2979.abstract
AB Conventional cytogenetic analysis of chromosome abnormalities in hematologic malignancies is hampered by the low mitotic index and poor quality of metaphases. A range of techniques based on fluorescence in situ hybridization (FISH) has greatly enhanced the identification of non-random translocations and deletions, pinpointing regions which contain genes involved in leukemogenesis. One of the main advantages of FISH is its ability to use non-dividing interphase cells as DNA targets, enabling the screening of large numbers of cells and providing access to a variety of cells with different hematopoetic activity. Furthermore, multicolor FISH (SKY, M-FISH and CGH microarrays) combines the screening potential of cytogenetics with the accuracy of molecular genetics, allowing the visualization of the entire human genome in 24 different colors. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved