TY - JOUR T1 - Impact of Cytogenetic and Molecular Cytogenetic Studies on Hematologic Malignancies JF - Anticancer Research JO - Anticancer Res SP - 2979 LP - 2983 VL - 25 IS - 4 AU - A. KOLIALEXI AU - G.TH. TSANGARIS AU - S. KITSIOU AU - E. KANAVAKIS AU - A. MAVROU Y1 - 2005/07/01 UR - http://ar.iiarjournals.org/content/25/4/2979.abstract N2 - Conventional cytogenetic analysis of chromosome abnormalities in hematologic malignancies is hampered by the low mitotic index and poor quality of metaphases. A range of techniques based on fluorescence in situ hybridization (FISH) has greatly enhanced the identification of non-random translocations and deletions, pinpointing regions which contain genes involved in leukemogenesis. One of the main advantages of FISH is its ability to use non-dividing interphase cells as DNA targets, enabling the screening of large numbers of cells and providing access to a variety of cells with different hematopoetic activity. Furthermore, multicolor FISH (SKY, M-FISH and CGH microarrays) combines the screening potential of cytogenetics with the accuracy of molecular genetics, allowing the visualization of the entire human genome in 24 different colors. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -