RT Journal Article
SR Electronic
T1 <em>In Vitro</em> Anticancer Activity of Cyanidin-3-<em>O</em>-β-Glucopyranoside: Effects on Transformed and Non-transformed T Lymphocytes
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2837
OP 2840
VO 25
IS 4
A1 FIMOGNARI, CARMELA
A1 BERTI, FAUSTO
A1 NÜSSE, MICHAEL
A1 CANTELLI-FORTI, GIORGIO
A1 HRELIA, PATRIZIA
YR 2005
UL http://ar.iiarjournals.org/content/25/4/2837.abstract
AB Background: Little is known regarding the potentially chemopreventive activity of cyanidin-3-O-β-glucopyranoside (Cy-g), the main anthocyanin present in the juice of pigmented oranges, apart from its antioxidant activity. After excluding a potential genotoxicity of Cy-g, its ability to induce apoptosis on transformed and normal T cells was analysed. In order to delineate the events leading to apoptosis, the expression of different proteins, known to be involved in apoptosis, was also measured. Materials and Methods: The evaluation of genotoxicity was performed by the micronucleus test. Flow cytometry was used for the analysis of apoptotic cells and proteins involved in the modulation of apoptosis. Results: Cy-g was nongenotoxic. Moreover, it induced apoptosis in both cell systems, modulated by an increase of p53 and bax proteins. Conclusion: These interesting biological properties should encourage further studies into the chemopreventive potential of Cy-g. Nevertheless, its activity in normal T cells underlines the need for extensive toxicological investigation. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved