PT  - JOURNAL ARTICLE
AU  - FIMOGNARI, CARMELA
AU  - BERTI, FAUSTO
AU  - NÜSSE, MICHAEL
AU  - CANTELLI-FORTI, GIORGIO
AU  - HRELIA, PATRIZIA
TI  - <em>In Vitro</em> Anticancer Activity of Cyanidin-3-<em>O</em>-β-Glucopyranoside: Effects on Transformed and Non-transformed T Lymphocytes
DP  - 2005 Jul 01
TA  - Anticancer Research
PG  - 2837--2840
VI  - 25
IP  - 4
4099  - http://ar.iiarjournals.org/content/25/4/2837.short
4100  - http://ar.iiarjournals.org/content/25/4/2837.full
SO  - Anticancer Res2005 Jul 01; 25
AB  - Background: Little is known regarding the potentially chemopreventive activity of cyanidin-3-O-β-glucopyranoside (Cy-g), the main anthocyanin present in the juice of pigmented oranges, apart from its antioxidant activity. After excluding a potential genotoxicity of Cy-g, its ability to induce apoptosis on transformed and normal T cells was analysed. In order to delineate the events leading to apoptosis, the expression of different proteins, known to be involved in apoptosis, was also measured. Materials and Methods: The evaluation of genotoxicity was performed by the micronucleus test. Flow cytometry was used for the analysis of apoptotic cells and proteins involved in the modulation of apoptosis. Results: Cy-g was nongenotoxic. Moreover, it induced apoptosis in both cell systems, modulated by an increase of p53 and bax proteins. Conclusion: These interesting biological properties should encourage further studies into the chemopreventive potential of Cy-g. Nevertheless, its activity in normal T cells underlines the need for extensive toxicological investigation. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved