RT Journal Article
SR Electronic
T1 Cytokeratin Expression Correlates with Aneuploidy in Cytological Specimens of Melanoma Metastases
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2789
OP 2792
VO 25
IS 4
A1 MONIKA KORABIOWSKA
A1 ILKA RUSCHENBURG
A1 HARALD SCHULZ
A1 ANJA STEINACKER
A1 PAWEL BORTKIEWICZ
A1 MICHAEL SEVENICH
A1 ULRICH BRINCK
A1 CARLOS CORDON-CARDO
A1 GÖSTA FISCHER
YR 2005
UL http://ar.iiarjournals.org/content/25/4/2789.abstract
AB It has been postulated that the high malignancy of melanomas could be connected with an increased cytokeratin (CK) expression. In order to define the relationship between CK expression and genetic instability of melanoma metastases, ploidy-related parameters were compared in cytological specimens of CK-positive and CK-negative melanoma metastases. CK expression was investigated immunohistochemically in 35 melanoma liver metastases and in 52 melanoma lymphatic metastases. Ploidy-related parameters were evaluated on Feulgen-stained specimens with a CAS200 image analyzer. Cytokeratin was detected in 14 out of 35 melanoma liver metastases and in 24 out of 52 melanoma lymphatic metastases investigated. Significant differences between CK-positive and CK-negative melanoma metastases were found for the percentage of diploid cells, percentage of tetraploid cells, percentage of aneuploid cells between 4c and 8c, as well as for 5c exceeding rate. Our results confirmed that CK is present in more advanced (unstable), clearly aneuploid forms of melanoma metastases. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved