TY - JOUR T1 - Microsatellite Status and Immunohistochemical Features of Ovarian Clear-cell Carcinoma JF - Anticancer Research JO - Anticancer Res SP - 2785 LP - 2788 VL - 25 IS - 4 AU - HARUHIKO UEDA AU - YOH WATANABE AU - HIDEKATSU NAKAI AU - HIROMICHI HEMMI AU - MINORU KOI AU - HIROSHI HOSHIAI Y1 - 2005/07/01 UR - http://ar.iiarjournals.org/content/25/4/2785.abstract N2 - Background: Ovarian clear-cell carcinoma (OCC) is known to have a poor prognosis and selected genetic features of OCC remain unknown. We investigated microsatellite instability (MSI) and the expression of the DNA mismatch repair-related protein, p53. Materials and Methods: MSI was examined by polymerase chain reaction using mono-, di-, tri- and tetranucleotide repeat markers, and hMSH2, hMLH1, hMSH6, MSH3 and p53 were determined immunohistochemically in 24 cases of OCC. Results: A total of 9 (37.5%) cases exhibited MSI. Two cases (8.3%) exhibited MSI-H in mononucleotide repeat loci with the negative expression of hMLH1, while another 7 cases (29.2%) exhibited selected trinucloetide repeat MSI (MSI-TR). Of these MSI-TR cases, 4 cases (57.1%) were determined to be negative for MSH3, while hMSH2, hMSH6, MSH3 and p53 expressions were normal. Conclusion: Our findings suggest that MSI-TR would be a feature indicating the microsatellite status in OCC, and that the loss of MSH3 expression may promote MSI-TR. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -