PT - JOURNAL ARTICLE AU - HARUHIKO UEDA AU - YOH WATANABE AU - HIDEKATSU NAKAI AU - HIROMICHI HEMMI AU - MINORU KOI AU - HIROSHI HOSHIAI TI - Microsatellite Status and Immunohistochemical Features of Ovarian Clear-cell Carcinoma DP - 2005 Jul 01 TA - Anticancer Research PG - 2785--2788 VI - 25 IP - 4 4099 - http://ar.iiarjournals.org/content/25/4/2785.short 4100 - http://ar.iiarjournals.org/content/25/4/2785.full SO - Anticancer Res2005 Jul 01; 25 AB - Background: Ovarian clear-cell carcinoma (OCC) is known to have a poor prognosis and selected genetic features of OCC remain unknown. We investigated microsatellite instability (MSI) and the expression of the DNA mismatch repair-related protein, p53. Materials and Methods: MSI was examined by polymerase chain reaction using mono-, di-, tri- and tetranucleotide repeat markers, and hMSH2, hMLH1, hMSH6, MSH3 and p53 were determined immunohistochemically in 24 cases of OCC. Results: A total of 9 (37.5%) cases exhibited MSI. Two cases (8.3%) exhibited MSI-H in mononucleotide repeat loci with the negative expression of hMLH1, while another 7 cases (29.2%) exhibited selected trinucloetide repeat MSI (MSI-TR). Of these MSI-TR cases, 4 cases (57.1%) were determined to be negative for MSH3, while hMSH2, hMSH6, MSH3 and p53 expressions were normal. Conclusion: Our findings suggest that MSI-TR would be a feature indicating the microsatellite status in OCC, and that the loss of MSH3 expression may promote MSI-TR. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved