PT  - JOURNAL ARTICLE
AU  - RAJENDRA GHARBARAN
AU  - CUI SHI
AU  - ONYEKWERE ONWUMERE
AU  - STEPHEN REDENTI
TI  - Plumbagin Induces Cytotoxicity <em>via</em> Loss of Mitochondrial Membrane Potential and Caspase Activation in Metastatic Retinoblastoma
AID  - 10.21873/anticanres.15287
DP  - 2021 Oct 01
TA  - Anticancer Research
PG  - 4725--4732
VI  - 41
IP  - 10
4099  - http://ar.iiarjournals.org/content/41/10/4725.short
4100  - http://ar.iiarjournals.org/content/41/10/4725.full
SO  - Anticancer Res2021 Oct 01; 41
AB  - Background/Aim: We investigated the cytotoxic effects of plumbagin on metastatic retinoblastoma, using the highly metastatic cell line Y79. Materials and Methods: Effect of plumbagin on cell growth was assessed with water-soluble tetrazolium 1 (WST-1) cell proliferation assay and automated hemocytometry with trypan blue-exclusion assay. Cell death was studied with acridine orange/ethidium bromide live-dead assay and annexin-V-fluorescein isothiocyanate/propidium iodide microscopy. Loss of mitochondrial membrane potential was studied with JC-10 dye and caspase activation was investigated using CellEvent Caspase-3/7 Green detection reagent. Results: Plumbagin highly significantly reduced the growth of Y79 cells treated for 24 h with 2.5 μM or more. Plumbagin also induced significantly high levels of cell death which was associated with loss of mitochondrial membrane potential and caspase activation. Conclusion: At very low concentration (2.5 μM), plumbagin potently induced cytotoxicity in metastatic retinoblastoma cells via loss of mitochondrial membrane potential and caspase activation.