RT Journal Article
SR Electronic
T1 Gene Expression Profiling in Chemoresistant Variants of Three Cell Lines of Different Origin
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2661
OP 2668
VO 25
IS 4
A1 JOHNSSON, ANDERS
A1 VALLON-CHRISTENSSON, JOHAN
A1 STRAND, CARINA
A1 LITMAN, THOMAS
A1 ERIKSEN, JENS
YR 2005
UL http://ar.iiarjournals.org/content/25/4/2661.abstract
AB Background: Drug resistance is a major problem in clinical cancer chemotherapy. Several mechanisms of resistance have been identified, but the underlying genomic changes are still poorly understood. Materials and Methods: Gene expression profiling, using cDNA microarray, was performed in eight cell lines (K562 leukemia, MCF-7 breast cancer and S1 colon cancer) with acquired resistance against five cytostatic drugs; daunorubicin (DNR), doxorubicin (DOX), vincristine (VCR), etoposide (VP) and mitoxantrone (MX). Results: The resistant cell lines clustered together based on their type of origin. Several genes encoding ABC transporters were highly up-regulated, most notably ABCB1 (MDR1) and ABCB4 in several cell lines and ABCG2 (MXR) specifically in MX-resistant cell lines. A pronounced down-regulation of several histones was noted in the MCF-7-derived resistant sublines. Altered expression was also seen in, e.g., GSTs, topoisomerases, caveolins, annexins and CD44. Conclusion: These results will constitute a platform for further studies on specific pathways and biological processes involved in chemotherapy resistance. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved