PT  - JOURNAL ARTICLE
AU  - JOHNSSON, ANDERS
AU  - VALLON-CHRISTENSSON, JOHAN
AU  - STRAND, CARINA
AU  - LITMAN, THOMAS
AU  - ERIKSEN, JENS
TI  - Gene Expression Profiling in Chemoresistant Variants of Three Cell Lines of Different Origin
DP  - 2005 Jul 01
TA  - Anticancer Research
PG  - 2661--2668
VI  - 25
IP  - 4
4099  - http://ar.iiarjournals.org/content/25/4/2661.short
4100  - http://ar.iiarjournals.org/content/25/4/2661.full
SO  - Anticancer Res2005 Jul 01; 25
AB  - Background: Drug resistance is a major problem in clinical cancer chemotherapy. Several mechanisms of resistance have been identified, but the underlying genomic changes are still poorly understood. Materials and Methods: Gene expression profiling, using cDNA microarray, was performed in eight cell lines (K562 leukemia, MCF-7 breast cancer and S1 colon cancer) with acquired resistance against five cytostatic drugs; daunorubicin (DNR), doxorubicin (DOX), vincristine (VCR), etoposide (VP) and mitoxantrone (MX). Results: The resistant cell lines clustered together based on their type of origin. Several genes encoding ABC transporters were highly up-regulated, most notably ABCB1 (MDR1) and ABCB4 in several cell lines and ABCG2 (MXR) specifically in MX-resistant cell lines. A pronounced down-regulation of several histones was noted in the MCF-7-derived resistant sublines. Altered expression was also seen in, e.g., GSTs, topoisomerases, caveolins, annexins and CD44. Conclusion: These results will constitute a platform for further studies on specific pathways and biological processes involved in chemotherapy resistance. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved