PT - JOURNAL ARTICLE AU - HUNG-YI CHEN AU - MEI-DUE YANG AU - YU-CHENG CHOU AU - YI-SHIH MA AU - SHU-FEN PENG AU - CHING-LUNG LIAO AU - PO-YUAN CHEN AU - TE-CHUN HSIA AU - JIN-CHERNG LIEN AU - CHUNG-HUNG CHEN TI - Ouabain Suppresses Cell Migration and Invasion in Human Gastric Cancer AGS Cells Through the Inhibition of MMP Signaling Pathways AID - 10.21873/anticanres.15241 DP - 2021 Sep 01 TA - Anticancer Research PG - 4365--4375 VI - 41 IP - 9 4099 - http://ar.iiarjournals.org/content/41/9/4365.short 4100 - http://ar.iiarjournals.org/content/41/9/4365.full SO - Anticancer Res2021 Sep 01; 41 AB - Background/Aim: Ouabain has been shown to induce human cancer cell death via apoptosis. Still, its anti-metastatic effect on cell migration and invasion of human gastric cancer cells has not been addressed. Materials and Methods: Cell proliferation and viability were measured by the MTT assay and flow cytometry, respectively. Cell motitlity was analysed by wound healing assay. Cell migration and invasion were analysed by the transwell system. Protein expression was assayed by western blotting. Results: Ouabain decreased AGS cell proliferation, cell viability, and motility. In addition, ouabain inhibited AGS cell migration and invasion. Furthermore, ouabain decreased matrix metalloproteinase-2 (MMP-2) activity at 48 h. Ouabain reduced the levels of proteins associated with PI3K/AKT and p38/MAPK pathways. In addition, ouabain decreased the expressions of N-cadherin, tissue inhibitor of metalloproteinases-1 (TIMP-1), urokinase-type plasminogen activator (c-uPA), and MMP-2 at 48 h. Conclusion: Ouabain suppresses cell metastasis through multiple signaling pathways in AGS cells.