TY - JOUR T1 - Allyl Isothiocyanate Induces DNA Damage and Impairs DNA Repair in Human Breast Cancer MCF-7 Cells JF - Anticancer Research JO - Anticancer Res SP - 4343 LP - 4351 DO - 10.21873/anticanres.15239 VL - 41 IS - 9 AU - CHING-LUNG LIAO AU - SHU-FEN PENG AU - JAW-CHYUN CHEN AU - PO-YUAN CHEN AU - AN-CHENG HUANG AU - JIN-CHERNG LIEN AU - FU-SHIN CHUEH AU - TAI-AN CHIANG AU - PING-PING WU AU - KUN-I LIN Y1 - 2021/09/01 UR - http://ar.iiarjournals.org/content/41/9/4343.abstract N2 - Background/Aim: Ally lisothiocyanate (AITC), a constituent of naturally occurring isothiocyanates (ITCs) found in some Brassica vegetables, has been previously demonstrated to have anti-carcinogenic activity. However, there is no available information showing that AITC induces DNA damage and alters DNA damage repair proteins in human breast cancer MCF-7 cells. Materials and Methods: In the present study, we investigated the effects of AITC on DNA damage and repair responses in human breast cancer MCF-7 cells in vitro. Cell viability was measured by flow cytometric assay. DNA condensation (apoptotic cell death) and DNA fragmentation (laddered DNA) were assayed by DAPI staining and DNA gel electrophoresis assays, respectively. Furthermore, DNA damage (comet tail) was measured by the comet assay. Western blotting was used to measure the expression of DNA damage- and repair-associated proteins. Results: AITC decreased cell viability in a dose-dependent and induced apoptotic cell death (DNA condensation and fragmentation) and DNA damage in MCF-7 cells. AITC increased p-ATMSer1981, p-ATRSer428, p53, p-p53Ser15, p-H2A.XSer139, BRCA1, and PARP at 10-30 μM at 24 and 48 h treatments. However, AITC decreased DNA-PK at 24 and 48 h treatment, and decreased MGMT at 48 h in MCF-7 cells. Conclusion: AITC induced cytotoxic effects (decreased viable cell number) through induction of DNA damage and condensation and altered DNA damage and repair associated proteins in MCF-7 cells in vitro. ER -