RT Journal Article
SR Electronic
T1 Allyl Isothiocyanate Induces DNA Damage and Impairs DNA Repair in Human Breast Cancer MCF-7 Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4343
OP 4351
DO 10.21873/anticanres.15239
VO 41
IS 9
A1 CHING-LUNG LIAO
A1 SHU-FEN PENG
A1 JAW-CHYUN CHEN
A1 PO-YUAN CHEN
A1 AN-CHENG HUANG
A1 JIN-CHERNG LIEN
A1 FU-SHIN CHUEH
A1 TAI-AN CHIANG
A1 PING-PING WU
A1 KUN-I LIN
YR 2021
UL http://ar.iiarjournals.org/content/41/9/4343.abstract
AB Background/Aim: Ally lisothiocyanate (AITC), a constituent of naturally occurring isothiocyanates (ITCs) found in some Brassica vegetables, has been previously demonstrated to have anti-carcinogenic activity. However, there is no available information showing that AITC induces DNA damage and alters DNA damage repair proteins in human breast cancer MCF-7 cells. Materials and Methods: In the present study, we investigated the effects of AITC on DNA damage and repair responses in human breast cancer MCF-7 cells in vitro. Cell viability was measured by flow cytometric assay. DNA condensation (apoptotic cell death) and DNA fragmentation (laddered DNA) were assayed by DAPI staining and DNA gel electrophoresis assays, respectively. Furthermore, DNA damage (comet tail) was measured by the comet assay. Western blotting was used to measure the expression of DNA damage- and repair-associated proteins. Results: AITC decreased cell viability in a dose-dependent and induced apoptotic cell death (DNA condensation and fragmentation) and DNA damage in MCF-7 cells. AITC increased p-ATMSer1981, p-ATRSer428, p53, p-p53Ser15, p-H2A.XSer139, BRCA1, and PARP at 10-30 μM at 24 and 48 h treatments. However, AITC decreased DNA-PK at 24 and 48 h treatment, and decreased MGMT at 48 h in MCF-7 cells. Conclusion: AITC induced cytotoxic effects (decreased viable cell number) through induction of DNA damage and condensation and altered DNA damage and repair associated proteins in MCF-7 cells in vitro.