PT  - JOURNAL ARTICLE
AU  - HEE KYUNG KIM
AU  - YAEWON YANG
AU  - SEONGGYU BYEON
AU  - YUSOOK JEONG
AU  - JIHYUN KWON
AU  - KI HYEONG LEE
AU  - SEUNG-MYOUNG SON
AU  - HYE SOOK HAN
TI  - E-Cadherin and Angiopoietin-2 as Potential Biomarkers for Colorectal Cancer With Peritoneal Carcinomatosis
AID  - 10.21873/anticanres.15260
DP  - 2021 Sep 01
TA  - Anticancer Research
PG  - 4497--4504
VI  - 41
IP  - 9
4099  - http://ar.iiarjournals.org/content/41/9/4497.short
4100  - http://ar.iiarjournals.org/content/41/9/4497.full
SO  - Anticancer Res2021 Sep 01; 41
AB  - Background/Aim: E-Cadherin has been implicated in cell-cell adhesion, and soluble E-cadherin is involved in angiogenesis and resistance to anti-angiogenic therapy in several cancer types. This study aimed to investigate the expression and clinical significance of soluble E-cadherin and other angiogenesis-related factors in plasma and malignant ascites of colorectal cancer (CRC) in patients with peritoneal carcinomatosis (PC). Materials and Methods: Multiplex enzyme-linked immunosorbent assay was performed on 95 body fluid samples (57 plasma and 38 malignant ascites) from patients with CRC. The status of E-cadherin and angiopoietin-2 (AGNPT2) was retrospectively evaluated by immunohistochemistry in primary CRC and paired metastatic peritoneal tissues or cell blocks of malignant ascites of 30 patients with peritoneal metastases of CRC. Results: The expression levels of soluble E-cadherin and ANGPT2 in plasma samples were significantly increased in patients with PC compared with those without. E-Cadherin concentration was significantly lower and ANGPT2 concentration was significantly higher in malignant ascites than plasma samples. Expression of E-cadherin was strongly positive, whilst that of ANGPT2 was negative in primary colorectal tissues, metastatic peritoneal tissues, and cell blocks of malignant ascites by immunohistochemistry. High levels of soluble E-cadherin or ANGPT2 in ascites were negatively associated with overall survival in patients with CRC with malignant ascites. Conclusion: Our findings suggest that soluble E-cadherin and ANGPT2 may be surrogate biomarkers for clinical outcome in patients with PC from CRC.