TY - JOUR T1 - Preclinical Activity of Plitidepsin Against Clear Cell Carcinoma of the Ovary JF - Anticancer Research JO - Anticancer Res SP - 4277 LP - 4285 DO - 10.21873/anticanres.15232 VL - 41 IS - 9 AU - RYUTA MIYAKE AU - SHOICHIRO YAMANAKA AU - SHO MATSUBARA AU - SEIJI MABUCHI Y1 - 2021/09/01 UR - http://ar.iiarjournals.org/content/41/9/4277.abstract N2 - Background/Aim: To evaluate the antitumor effects of Plitidepsin against clear cell carcinoma (CCC) of the ovary. Materials and Methods: The expression of eEF1A2 in ovarian cancer was assessed by immunohistochemistry. Using ovarian CCC cell lines, the antitumor effect of Plitidepsin was assessed both in vitro and in vivo. By over-expressing or knocking down the eEF1A2 expression, we investigated the role of eEF1A2 in the sensitivity of CCC cells to Plitidepsin. Results: Immunoreactivity to eEF1A2 was observed in 76.2% of CCC, which was significantly higher than other histological subtypes of ovarian cancer. Plitidepsin exhibited significant antitumor activity toward chemonaive and chemoresistant CCC cells both in vitro and in vivo. Ectopic expression of eEF1A2 in CCC cells resulted in increased sensitivity to Plitidepsin. In contrast, eEF1A2 knockdown decreased sensitivity of CCC cells to plitidepsin. Conclusion: Plitidepsin, a novel anti-cancer agent that targets eEF1A2, may be a promising agent for treating ovarian CCC. ER -