TY - JOUR T1 - Cancer Associated Fibroblasts Derived from Pancreatic Adenocarcinoma and Their Role in Cell Migration JF - Anticancer Research JO - Anticancer Res SP - 4229 LP - 4238 DO - 10.21873/anticanres.15227 VL - 41 IS - 9 AU - ELLEN VON HOERSCHELMANN AU - CHRISTOPHER C. M. NEUMANN AU - MATTHAUS FELSENSTEIN AU - JULIA GOGOLOK AU - ANJA REUTZEL-SELKE AU - IGOR M. SAUER AU - JOHANN PRATSCHKE AU - MARCUS BAHRA AU - ROSA B. SCHMUCK Y1 - 2021/09/01 UR - http://ar.iiarjournals.org/content/41/9/4229.abstract N2 - Background/Aim: Pancreatic ductal adenocarcinoma (PDAC) shows poor survival and early systemic dissemination. Cancer associated fibroblasts (CAFs) enhance migration and invasion of cancer cells. We aimed to investigate the role of CAFs in cell migration and their underlying paracrine effects. Materials and Methods: Using Transwell® migration assays, PDAC cells (PANC-1) and three distinct types of fibroblasts were analyzed: CAFs, genetically transformed human foreskin-fibroblasts (BJeLR), and non-transformed human foreskin-fibroblasts (VH7). IL6 in the culture supernatant was measured to investigate paracrine communication in monocultures and direct/indirect cocultures. Results: CAFs showed a significantly higher capacity to migrate in vitro when compared to benign fibroblasts (p=0.009). They also facilitated the migration of PDAC cells in coculture (p=0.001). Neither BJeLR, nor VH7 displayed such features. This was accompanied by a significant increase in IL-6 when CAFs were cocultured with PANC-1 (p=0.009). Conclusion: CAFs are a key element of intra-tumoral migration and should be further investigated as a potential therapeutic target. ER -