TY - JOUR T1 - Her-2/neu Expression in Breast Cancer - A Comparison of Different Diagnostic Methods JF - Anticancer Research JO - Anticancer Res SP - 1895 LP - 1900 VL - 25 IS - 3B AU - P. BENÖHR AU - V. HENKEL AU - R. SPEER AU - U. VOGEL AU - K. SOTLAR AU - B. AYDENIZ AU - A. REISER AU - H. NEUBAUER AU - K. TABITI AU - D. WALLWIENER AU - S.E. CLARE AU - R. KUREK Y1 - 2005/05/01 UR - http://ar.iiarjournals.org/content/25/3B/1895.abstract N2 - Background: Determination of Her-2/neu overexpression in breast cancer has previously been shown to be of prognostic significance. In this study, Her-2/neu expression in breast cancer was characterised by real-time PCR (RLT-PCR) based LightCycler-HER-2/neu DNA Quantification with immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH). Material and Methods: Fifteen specimens of invasive breast cancer - whole tissue sections as well as microdissected tumour cells - were subjected to RLT-PCR. Additionally, IHC and FISH were performed. Results: Her-2/neu overexpression was detected by FISH and by real-time PCR in the same tumours. In contrast, IHC revealed discordant results. Conclusion: Determination of Her-2/neu amplification by real-time PCR is a sensitive and specific method with some advantages over FISH. This method is simple and reliable and has the potential of categorizing those tumours with borderline Her-2/neu overexpression as determined by IHC. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -