RT Journal Article
SR Electronic
T1 Global Analysis of Protein Expression in A549 Cells After Prolonged Nicotine Exposure by Using Label-free Quantification
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3833
OP 3842
DO 10.21873/anticanres.15176
VO 41
IS 8
A1 SASIKARN KOMKLEOW
A1 CHURAT WEERAPHAN
A1 DARANEE CHOKCHAICHAMNANKIT
A1 PAPADA CHAISURIYA
A1 CHRIS VERATHAMJAMRAS
A1 THEETAT RUANGJAROON
A1 JISNUSON SVASTI
A1 POLKIT SANGVANICH
A1 CHANTRAGAN SRISOMSAP
YR 2021
UL http://ar.iiarjournals.org/content/41/8/3833.abstract
AB Background/Aim: Lung cancer is the leading cause of cancer death worldwide. Cigarette smoke is the most important risk factor for cancer development. Growing evidence indicates that prolonged nicotine exposure is a potential factor associated with tumorigenesis. Here, the effect of prolonged nicotine exposure on A549 cells was investigated, using label-free quantitative proteomics. Materials and Methods: Selection of an invasive subpopulation from the A549 cell line was performed to reveal the differential expression of proteins in relation to prolonged nicotine exposure, using Boyden chamber assays in combination with a proteomics approach. Results: One hundred proteins from the NicoA549-L5 subline showed significant change in expression compared to those from the A549-L5 subline and their A549 parental cell line. Heat shock protein, protein disulfide isomerase A3, profilin-1 and legumain were expressed at higher levels in A549 cells after prolonged nicotine exposure. Conclusion: These aberrant proteins might serve as novel cancer biomarkers for cigarette smokers.