PT  - JOURNAL ARTICLE
AU  - SASIKARN KOMKLEOW
AU  - CHURAT WEERAPHAN
AU  - DARANEE CHOKCHAICHAMNANKIT
AU  - PAPADA CHAISURIYA
AU  - CHRIS VERATHAMJAMRAS
AU  - THEETAT RUANGJAROON
AU  - JISNUSON SVASTI
AU  - POLKIT SANGVANICH
AU  - CHANTRAGAN SRISOMSAP
TI  - Global Analysis of Protein Expression in A549 Cells After Prolonged Nicotine Exposure by Using Label-free Quantification
AID  - 10.21873/anticanres.15176
DP  - 2021 Aug 01
TA  - Anticancer Research
PG  - 3833--3842
VI  - 41
IP  - 8
4099  - http://ar.iiarjournals.org/content/41/8/3833.short
4100  - http://ar.iiarjournals.org/content/41/8/3833.full
SO  - Anticancer Res2021 Aug 01; 41
AB  - Background/Aim: Lung cancer is the leading cause of cancer death worldwide. Cigarette smoke is the most important risk factor for cancer development. Growing evidence indicates that prolonged nicotine exposure is a potential factor associated with tumorigenesis. Here, the effect of prolonged nicotine exposure on A549 cells was investigated, using label-free quantitative proteomics. Materials and Methods: Selection of an invasive subpopulation from the A549 cell line was performed to reveal the differential expression of proteins in relation to prolonged nicotine exposure, using Boyden chamber assays in combination with a proteomics approach. Results: One hundred proteins from the NicoA549-L5 subline showed significant change in expression compared to those from the A549-L5 subline and their A549 parental cell line. Heat shock protein, protein disulfide isomerase A3, profilin-1 and legumain were expressed at higher levels in A549 cells after prolonged nicotine exposure. Conclusion: These aberrant proteins might serve as novel cancer biomarkers for cigarette smokers.