RT Journal Article SR Electronic T1 Effects of Kumaizasa (Sasa senanensis) Leaf Extract on Innate Immune Regulation in HEK293 Cells and Macrophages JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4093 OP 4100 DO 10.21873/anticanres.15212 VO 41 IS 8 A1 KEISUKE SATO A1 RYOSUKE TATSUNAMI A1 AKIFUMI NAKATA A1 KEN-ICHI KOMATSU A1 SHINJI HARAKAWA A1 TAKAKI NEDACHI A1 KAORU HAKETA A1 HIROYUKI INAGAWA A1 KOJI WAKAME YR 2021 UL http://ar.iiarjournals.org/content/41/8/4093.abstract AB Background/Aim: We investigated the effect of Kumaizasa leaf extract (KLE) on innate immunity using the HEK293 and RAW 264.7 cell lines. Materials and Methods: KLE, lipopolysaccharides (LPS), or KLE with LPS were added to RAW 264.7 cells. The TNF-α and IL-1β mRNA expression was then quantified. The expression of MAPKs, NFĸB, TNF-α and IL-1β proteins was also quantified. In addition, KLE was added to HEK293 cells and the IL-8 concentration was measured. Results: In RAW 264.7 cells, KLE increased the levels of TNF-α and IL-1β mRNA. By contrast, when KLE and LPS were added to RAW 264.7 cells, the increase in TNF-α and IL-1β mRNA was ameliorated. Similarly, the expression of JNK and ERK proteins was reduced. The addition of KLE to HEK293 cells induced IL-8 production. Conclusion: Based on these results, a KLE-mediated mechanism may regulate immunity by suppressing the expression of JNK and ERK, which are involved in inflammatory signal transduction.