TY - JOUR T1 - Effects of Kumaizasa (<em>Sasa senanensis</em>) Leaf Extract on Innate Immune Regulation in HEK293 Cells and Macrophages JF - Anticancer Research JO - Anticancer Res SP - 4093 LP - 4100 DO - 10.21873/anticanres.15212 VL - 41 IS - 8 AU - KEISUKE SATO AU - RYOSUKE TATSUNAMI AU - AKIFUMI NAKATA AU - KEN-ICHI KOMATSU AU - SHINJI HARAKAWA AU - TAKAKI NEDACHI AU - KAORU HAKETA AU - HIROYUKI INAGAWA AU - KOJI WAKAME Y1 - 2021/08/01 UR - http://ar.iiarjournals.org/content/41/8/4093.abstract N2 - Background/Aim: We investigated the effect of Kumaizasa leaf extract (KLE) on innate immunity using the HEK293 and RAW 264.7 cell lines. Materials and Methods: KLE, lipopolysaccharides (LPS), or KLE with LPS were added to RAW 264.7 cells. The TNF-α and IL-1β mRNA expression was then quantified. The expression of MAPKs, NFĸB, TNF-α and IL-1β proteins was also quantified. In addition, KLE was added to HEK293 cells and the IL-8 concentration was measured. Results: In RAW 264.7 cells, KLE increased the levels of TNF-α and IL-1β mRNA. By contrast, when KLE and LPS were added to RAW 264.7 cells, the increase in TNF-α and IL-1β mRNA was ameliorated. Similarly, the expression of JNK and ERK proteins was reduced. The addition of KLE to HEK293 cells induced IL-8 production. Conclusion: Based on these results, a KLE-mediated mechanism may regulate immunity by suppressing the expression of JNK and ERK, which are involved in inflammatory signal transduction. ER -