RT Journal Article
SR Electronic
T1 Construction of a Drug Release Evaluation System: Application of Mitochondrial Respiration to Monitor Drug Release
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4083
OP 4088
DO 10.21873/anticanres.15210
VO 41
IS 8
A1 KAZUTO OHKURA
A1 YOHEI TATEMATSU
A1 ATSUSHI TABATA
YR 2021
UL http://ar.iiarjournals.org/content/41/8/4083.abstract
AB Background/Aim: Efficient drug encapsulation and regulation of drug release are important factors for sustained drug release and application for release-controlled anti-cancer and anti-inflammatory drug delivery. In the present study, a direct evaluation system for drug-release from model carrier (e.g., alginate-gel beads) was examined using the mitochondrial oxygen consumption rate as an index. Materials and Methods: Alginate-gel beads were coated with the uncoupler SF6847 (SF beads) and used as a model microparticle-type drug. The real-time monitoring of SF6847 release from prepared alginate-gel beads was performed using the mitochondrial oxygen consumption rate. Release profiles of nonsteroidal anti-inflammatory drugs [NSAIDs, mefenamic acid (MEF) and diclofenac (DIC)] from alginate-gel beads as well as SF beads were investigated using the real time monitoring system. Results: SF6847 release from alginate-gel beads was clearly detected using the rat liver mitochondrial oxygen consumption rate. The release features of MEF and DIC from alginate-gel beads were defined by the present trial monitoring system, and these NSAIDs exhibited different release profiles. Conclusion: The present drug monitoring system detected released drugs, and the release profile reflected the molecular properties of the test drugs. This system may be applied to the design and development of precise sustained drug release systems (e.g., anti-cancer and anti-inflammatory drugs).