TY - JOUR T1 - Cancer Cell-specific Transfection of hCas9 Gene Using Ad5F35 Vector JF - Anticancer Research JO - Anticancer Res SP - 3731 LP - 3740 DO - 10.21873/anticanres.15164 VL - 41 IS - 8 AU - WATARU MATSUNAGA AU - KATSUYUKI HAMADA AU - MASATOSHI TAGAWA AU - TAKAO MORINAGA AU - AKINOBU GOTOH Y1 - 2021/08/01 UR - http://ar.iiarjournals.org/content/41/8/3731.abstract N2 - Background: The clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) is thought to have promising clinical potential. However, the off-target effects of Cas9 are a major concern for its application. Therefore, we hypothesized that the adverse effects of off-target gene editing might be minimized if the human codon-optimized Streptococcus pyogenes Cas9 (hCas9) could be specifically expressed in cancer cells. Materials and Methods: We constructed a chimeric adenoviral vector, Ad5F35-MKp-hCas9, and infected human bladder cancer cell lines with this vector. The confirmation of hCas9 gene expression was performed in 3-4 days after from infection. Results: hCas9 gene expression was observed in Ad5F35-MKp-hCas9 infected bladder cancer cells but not in non-malignant cells. Conclusion: Our study showed that the Ad5F35-MKp-hCas9 vector is capable of expressing the hCas9 gene with high specificity in bladder cancer cells. These findings may help in minimizing the risk of off-target effects of gene editing. ER -