%0 Journal Article %A CHIE MAEDA %A AKIRA IIZUKA %A HARUO MIYATA %A RYOTA KONDOU %A TADASHI ASHIZAWA %A AKARI KANEMATSU %A KYOKO WATANABE %A SHOICHI DEGUCHI %A KOICHI MITSUYA %A NAKAMASA HAYASHI %A YOSHIAKI ABE %A KEN YAMAGUCHI %A YASUTO AKIYAMA %T Alternative Evaluation of an ELISPOT Assay Using Cytokine Activity as a Novel Parameter %D 2021 %R 10.21873/anticanres.15175 %J Anticancer Research %P 3825-3831 %V 41 %N 8 %X Background/Aim: The enzyme-linked immunospot (ELISPOT) assay is a well-established method used to evaluate the strength of T cell-mediated immune activity, and accepted as a standard functional immunological assay. Cytokine activity is a novel parameter reflecting spot size and intensity, which has not been used in ELISPOT assay before. Materials and Methods: In the present study, from 113 ELISPOT assay data derived from previous clinical trials with dendritic cell vaccines, both spot number count and cytokine activity data for IFN-γ secretion were obtained using an ELISPOT reader. Comparing the new parameter cytokine activity with the existing parameter spot number, the feasibility of cytokine activity was investigated. Results: There were no significant differences in sensitivity and specificity between spot number and cytokine activity among ELISPOT assay data from CMVpp65 and other antigen peptide-stimulated cytotoxic T lymphocytes. Conclusion: Although cytokine activity is a novel parameter unreported so far, it did not show any advantages in the evaluation T cell immune responses compared to the existing spot number parameter. %U https://ar.iiarjournals.org/content/anticanres/41/8/3825.full.pdf