RT Journal Article SR Electronic T1 Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4089 OP 4092 DO 10.21873/anticanres.15211 VO 41 IS 8 A1 KUMPEI KAWAKATSU A1 MISATO MAEMURA A1 YOSHIKA SETA A1 TAKAHITO NISHIKATA YR 2021 UL http://ar.iiarjournals.org/content/41/8/4089.abstract AB Background/Aim: Serum-derived macrophage activating factor (serum-MAF) is expected to have adjuvant effects through rapid phagocytic activation, which depends on F-actin accumulation in multi-layered membrane ruffles induced within 5 min after serum-MAF addition. This study aimed to elucidate the importance of annexin A2, which is a multifunctional Ca2+-binding protein related to cytoskeletal membrane dynamics, in serum-MAF signalling. Materials and Methods: Annexin A2 and F-actin localizations were analyzed via immunostaining and confocal microscopy. Using EGTA as chelator, the role of Ca2+ in serum-MAF signalling was examined. Results: Annexin A2 was found to translocate from the cytosol to the cell cortex within 30 s of serum-MAF stimulation. Ca2+ chelation inhibited the translocation of annexin A2, frill-like structure formation, and phagocytic activation by serum-MAF. Conclusion: Annexin A2 and Ca2+ were responsible for the rapid phagocytic activation by serum-MAF. This study provides an understanding of phagocytic activation in macrophages, which could be beneficial for cancer immunotherapy.