PT  - JOURNAL ARTICLE
AU  - KUMPEI KAWAKATSU
AU  - MISATO MAEMURA
AU  - YOSHIKA SETA
AU  - TAKAHITO NISHIKATA
TI  - Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages
AID  - 10.21873/anticanres.15211
DP  - 2021 Aug 01
TA  - Anticancer Research
PG  - 4089--4092
VI  - 41
IP  - 8
4099  - http://ar.iiarjournals.org/content/41/8/4089.short
4100  - http://ar.iiarjournals.org/content/41/8/4089.full
SO  - Anticancer Res2021 Aug 01; 41
AB  - Background/Aim: Serum-derived macrophage activating factor (serum-MAF) is expected to have adjuvant effects through rapid phagocytic activation, which depends on F-actin accumulation in multi-layered membrane ruffles induced within 5 min after serum-MAF addition. This study aimed to elucidate the importance of annexin A2, which is a multifunctional Ca2+-binding protein related to cytoskeletal membrane dynamics, in serum-MAF signalling. Materials and Methods: Annexin A2 and F-actin localizations were analyzed via immunostaining and confocal microscopy. Using EGTA as chelator, the role of Ca2+ in serum-MAF signalling was examined. Results: Annexin A2 was found to translocate from the cytosol to the cell cortex within 30 s of serum-MAF stimulation. Ca2+ chelation inhibited the translocation of annexin A2, frill-like structure formation, and phagocytic activation by serum-MAF. Conclusion: Annexin A2 and Ca2+ were responsible for the rapid phagocytic activation by serum-MAF. This study provides an understanding of phagocytic activation in macrophages, which could be beneficial for cancer immunotherapy.