RT Journal Article
SR Electronic
T1 Activity and Therapeutic Potential of ORI-1001 Antisense Oligonucleotide on Human Papillomavirus Replication Utilizing a Model of Dysplastic Human Epithelium
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 765
OP 777
VO 25
IS 2A
A1 ALAM, SAMINA
A1 BROMBERG-WHITE, JENNIFER
A1 MCLAUGHLIN-DRUBIN, MARGARET
A1 SEN, ELLORA
A1 M. BODILY, JASON
A1 MEYERS, CRAIG
YR 2005
UL http://ar.iiarjournals.org/content/25/2A/765.abstract
AB Human Papillomaviruses (HPVs) are small double-stranded DNA viruses that infect the cutaneous or mucosal epithelium. The high-risk genital HPVs are associated with squamous intraepithelial lesions of the anogenital region that can progress to cancer. Cervical cancer is the third leading cause of cancer death in women worldwide, yet there are no specific therapeutic treatments for HPV-associated malignancies. Development of specific antisense oligonucleotides as antiviral agents is an alternative therapeutic strategy. We utilized the organotypic raft culture system which recapitulates the entire HPV life cycle, including the production of infectious virions. We studied the effect of the ORI-1001 antisense phosphorothioate oligonucleotide designed against the E1 mRNA translation start site of low-risk HPV6 and HPV11, and tested it against high-risk HPV31b and HPV16 vegetative replication and oncogene promoter activity. ORI-1001 significantly inhibited HPV31b genome amplification. In contrast, HPV16 genome amplification was unaffected. In addition, ORI-1001 significantly downregulated transcriptional activity from a HPV31b p99 early promoter luciferase reporter construct, and inhibited E1 and E6E7 transcript expression from the wild-type genome. Our results support the idea that the antisense activity of ORI-1001 can target HPV31b functional activities in the differentiation dependent life cycle of this virus. Our results predict that binding stability between antisense oligonucleotides with partial homology to HPV genes may mediate targeting of multiple HPV types. Our studies also highlight the utility of the raft culture system in defining the parameters for testing antisense oligonucleotides against HPV. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved