RT Journal Article
SR Electronic
T1 Heparanase Expression Is Associated With Cancer Stem Cell Features and Radioresistance in Hodgkin’s Lymphoma Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3299
OP 3308
DO 10.21873/anticanres.15117
VO 41
IS 7
A1 TROSCHEL, FABIAN M.
A1 LINSENMAIER, MATTHIAS
A1 BORRMANN, KATRIN
A1 EICH, HANS THEODOR
A1 GÖTTE, MARTIN
A1 GREVE, BURKHARD
YR 2021
UL http://ar.iiarjournals.org/content/41/7/3299.abstract
AB Background/aim: Heparanase (HPSE) is relevant to therapy resistance in many malignancies yet is largely unstudied in Hodgkin’s lymphoma. Here, we investigated links between HPSE, cancer stem cell (CSC) features and radioresistance in KM-H2 and L428 Hodgkin’s lymphoma cells. Materials and Methods: Firstly, HPSE expression in unsorted and sorted CSCs was assessed. Post-irradiation, HPSE and CSC-related gene expression changes were then quantified. Clonogenic ability was investigated with and without artificial changes in HPSE expression pre and post irradiation. Results: HPSE was highly expressed in L428 but barely present in KM-H2 cells. HPSE was overexpressed in sorted L428 CSCs. Irradiation induced HPSE and expression of CSC markers. High HPSE-expressing L428 cells showed higher clonogenic ability than low HPSE-expressing KM-H2 cells after irradiation. Down-regulation of HPSE in L428 cells reduced their clonogenic capability post-radiation, whilst overexpression of HPSE in KM-H2 cells increased colony formation. Conclusion: HPSE expression is associated with CSC features and contributes to radioresistance in Hodgkin’s lymphoma cells.