RT Journal Article
SR Electronic
T1 Quantitative and Functional Assay of MDR1/P170-mediated MDR in Ascites Cells of Patients with Ovarian Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1187
OP 1192
VO 25
IS 2A
A1 KRASZNAI, ZOARD TIBOR
A1 FRIEDLÄNDER, ELZA
A1 NAGY, ANDRAS
A1 SZABO, GABOR
A1 VEREB, GYÖRGY
A1 GODA, KATALIN
A1 HERNADI, ZOLTAN
YR 2005
UL http://ar.iiarjournals.org/content/25/2A/1187.abstract
AB Background: MDR1-associated P-glycoproteindependent multidrug resistance is a common cause of chemotherapy failure in patients with advanced ovarian cancer. Here, we describe a clinical method for simultaneously assessing the expression and function of the MDR1/Pgp in tumour cells from ascites of patients with malignant ovarian carcinoma. Materials and Methods: Cells from ascites from 35 patients were collected. The expression and function of Pgp were detected by flow cytometry. For functional study, rhodamine 123 was used. Results: Using the Pgp- specific UIC2 and MM6.15 antibodies, we demonstrated the presence of Pgp in 10-79 % (38.9±20, 7; n=35) of the CA125-positive cell subpopulations. The results of the functional assay showed strong correlation with the level of Pgp expression (r=0.976; p=3.2×10-5). Conclusion: Direct detection of the expression level and function of MDR1/Pgp in the ascites provide useful information for the more efficient treatment of malignant diseases by proper adjustment of the chemotherapeutic protocol. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved