PT  - JOURNAL ARTICLE
AU  - PAULA M. DE ANGELIS
AU  - LINDA DORG
AU  - SEAN PHAM
AU  - SOLVEIG NORHEIM ANDERSEN
TI  - DNA Repair Protein Expression and Oxidative/Nitrosative Stress in Ulcerative Colitis and Sporadic Colorectal Cancer
AID  - 10.21873/anticanres.15112
DP  - 2021 Jul 01
TA  - Anticancer Research
PG  - 3261--3270
VI  - 41
IP  - 7
4099  - http://ar.iiarjournals.org/content/41/7/3261.short
4100  - http://ar.iiarjournals.org/content/41/7/3261.full
SO  - Anticancer Res2021 Jul 01; 41
AB  - Background/Aim: Chronic inflammation generates large quantities of reactive oxygen and nitrogen species that damage DNA. DNA repair is important for cellular viability and genome integrity. Materials and Methods: Expression levels of the DNA repair proteins OGG1, XPA, MLH1, PARP1, and XRCC6, which function in base excision repair, nucleotide excision repair, mismatch repair, single-strand break repair and double-strand break repair, respectively, were assessed using immunohistochemistry in ulcerative colitis and sporadic colorectal cancer biopsies. Levels of oxidative/ nitrosative stress biomarkers were also assessed. Results: Ulcerative colitis and colorectal cancer lesions expressed significantly higher levels of all DNA repair proteins and oxidative/ nitrosative stress biomarkers compared to normal colonic mucosa. Ulcerative colitis had the highest XPA and XRCC6 expression. Conclusion: Oxidative/nitrosative stress is prevalent in the colon of both diseases. Nucleotide excision repair and non-homologous end-joining double-strand break repair may be compromised in colorectal cancer, but not in ulcerative colitis.