RT Journal Article
SR Electronic
T1 The Expression of p21/WAF-1 and Cyclin B1 Mediate Mitotic Delay in x-Irradiated Fibroblasts
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1123
OP 1129
VO 25
IS 2A
A1 CARIVEAU, MICKAEL J.
A1 KOVACS, CHARLES J.
A1 ALLISON, RON R.
A1 JOHNKE, ROBERTA M.
A1 KALMUS, GERHARD W.
A1 EVANS, MARK
YR 2005
UL http://ar.iiarjournals.org/content/25/2A/1123.abstract
AB Background: To better understand the relationship between mitotic delay and the disruption of cyclin B1 and p21 in x-irradiated fibroblasts, studies were carried out to establish correlations between the downregulation of cyclin B1 by the cyclin kinase inhibitor (CKI) p21 and the induction of mitotic delay in the NIH3T3 fibroblast. Materials and Methods: Cell cycle kinetics were used to analyze mitotic delay in irradiated NIH3T3 cells and immunocytochemistry incorporated to assess the expression of cyclin B1 and p21, following 2 or 4Gy x-irradiation. Results and Discussion: Results indicate a dose dependent increase in mitotic delay accompanied by a downregulation of cyclin B1 and corresponding upregulation of the CKI p21 in exponentially growing cultures. Data indicates that the induction of radiation - induced division delay appears to be dependent on the p21 inhibition of cyclin B1 and, furthermore, p21 and cyclin B1 expression are highly dependent on cell density. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved