TY - JOUR T1 - Jaspine B Hydrochloride-induced Apoptosis in HeLa Cells Is Associated With Disrupted Sphingolipid Metabolism and Ceramide Overload JF - Anticancer Research JO - Anticancer Res SP - 2875 LP - 2883 DO - 10.21873/anticanres.15069 VL - 41 IS - 6 AU - ALEXANDRA BOGDANOVA AU - MARTIN KELLO AU - ALEXANDRA MACEJOVA AU - NATALIA NOSALOVA AU - PETER PETIK AU - PETER TAKAC AU - MIROSLAVA MARTINKOVA AU - EVA MEZEIOVA AU - LADISLAV MIROSSAY AU - PETER GAL AU - MARTINA BAGO PILATOVA Y1 - 2021/06/01 UR - http://ar.iiarjournals.org/content/41/6/2875.abstract N2 - Background/Aim: A series of experiments on HeLa cells were conducted to provide new information concerning the anti-cancer properties of jaspine B hydrochloride (JBH). Materials and Methods: HeLa cells treated with 0.5 μmol/l JBH for 24, 48, and 72 h underwent flow cytometric analysis of the cell cycle, and measurement of phosphatidylserine externalization, mitochondrial membrane potential (MMP), casp-3 activation, cleavage of PARP, ceramide levels, aSMase activity, and Bcl-2 release. nSMase activity was measured by a colorimetric assay. Gene expression was determined by qRT–PCR. Immunocytochemistry was performed to detect p21 and p27 expression. Results: JBH-induced apoptosis in HeLa cells associated with externalization of phosphatidylserine, reduced MMP, activation of casp-3, and cleavage of PARP as well as up-regulation of TNF-α, FasL, and casp-8. Significant increase in nSMase activity, ceramide levels, Bcl-2 release (predominantly in the inactive form), and pro-apoptotic nuclear localization of p21 and p27 were also detected. Conclusion: JBH-induced apoptosis in HeLa cells is associated with disrupted sphingolipid homeostasis resulting in increased ceramide levels. ER -