RT Journal Article
SR Electronic
T1 Exosome Transfer Between Pancreatic-cancer Cells Is Associated With Metastasis in a Nude-mouse Model
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2829
OP 2834
DO 10.21873/anticanres.15063
VO 41
IS 6
A1 HIRONAO ICHIKAWA
A1 ATSUSHI SUETSUGU
A1 TOMOYUKI SATAKE
A1 HITOMI AOKI
A1 TAKAHIRO KUNISADA
A1 MASAHITO SHIMIZU
A1 ROBERT M. HOFFMAN
YR 2021
UL http://ar.iiarjournals.org/content/41/6/2829.abstract
AB Background/Aim: Cancer-derived exosomes play an important role in metastasis. In the present study, we determined whether exosome transfer between cancer cells is associated with metastasis in a mouse model. Materials and Methods: AsPC-1 human pancreatic-cancer cells expressing red fluorescent protein (RFP) and AsPC-1 human pancreatic-cancer cells transduced by exosome-specific pCT-CD63-green fluorescent protein (GFP), were co-injected into the spleen of nude mice. Results: Both pancreatic-cancer cell lines grew in the spleen and metastasized to the liver, peritoneum, and lungs, as shown by color-coded imaging. The ratio of GFP-expressing exosomes incorporated in RFP-labeled AsPC-1 cells was statistically-significantly higher in the liver, lung, and peritoneal metastases than in the spleen. Conclusion: Exosome transfer between cancer cells is associated with metastasis. Exosome transfer may play a role in increasing the metastatic capability of the recipient cells.