TY - JOUR T1 - Exosome Transfer Between Pancreatic-cancer Cells Is Associated With Metastasis in a Nude-mouse Model JF - Anticancer Research JO - Anticancer Res SP - 2829 LP - 2834 DO - 10.21873/anticanres.15063 VL - 41 IS - 6 AU - HIRONAO ICHIKAWA AU - ATSUSHI SUETSUGU AU - TOMOYUKI SATAKE AU - HITOMI AOKI AU - TAKAHIRO KUNISADA AU - MASAHITO SHIMIZU AU - ROBERT M. HOFFMAN Y1 - 2021/06/01 UR - http://ar.iiarjournals.org/content/41/6/2829.abstract N2 - Background/Aim: Cancer-derived exosomes play an important role in metastasis. In the present study, we determined whether exosome transfer between cancer cells is associated with metastasis in a mouse model. Materials and Methods: AsPC-1 human pancreatic-cancer cells expressing red fluorescent protein (RFP) and AsPC-1 human pancreatic-cancer cells transduced by exosome-specific pCT-CD63-green fluorescent protein (GFP), were co-injected into the spleen of nude mice. Results: Both pancreatic-cancer cell lines grew in the spleen and metastasized to the liver, peritoneum, and lungs, as shown by color-coded imaging. The ratio of GFP-expressing exosomes incorporated in RFP-labeled AsPC-1 cells was statistically-significantly higher in the liver, lung, and peritoneal metastases than in the spleen. Conclusion: Exosome transfer between cancer cells is associated with metastasis. Exosome transfer may play a role in increasing the metastatic capability of the recipient cells. ER -