RT Journal Article
SR Electronic
T1 DNA-binding Cell-penetrating Peptide-based TRAIL Over-expression in Adipose Tissue-derived Mesenchymal Stem Cells Inhibits Glioma U251MG Growth
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2859
OP 2866
DO 10.21873/anticanres.15067
VO 41
IS 6
A1 JAESIK SHIN
A1 SOON KOO BAIK
A1 YONGDAE YOON
A1 SOONJAE HWANG
A1 JOON HYUNG SOHN
A1 MINJEONG JO
A1 WOO-SEUNG KIM
A1 KI-JONG RHEE
A1 KUM WHANG
A1 YOUNG WOO EOM
YR 2021
UL http://ar.iiarjournals.org/content/41/6/2859.abstract
AB Background/Aim: Genetic manipulation of stem cells using non-viral vectors is still limited due to low transfection efficiency. We investigated whether the DNA-binding cell-permeation peptides (CPP) can enhance the transfection efficiency of non-viral vectors in adipose tissue-derived mesenchymal stem cells (ASCs) and whether ASCs over-expressing TRAIL through CPP can inhibit the growth of glioma U251MG cells in vitro and in vivo. Materials and Methods: ASCs were genetically engineered to over-express TRAIL by using CPP, pCMV3-TRAIL and lipid-based transfection reagents (X-tremeGENE). Results: The transfection efficiency of ASCs increased by approximately 7% using CPP; 53.9% of ASCs were transfected and TRAIL expression in ASCs increased by approximately 3 times compared to X-tremeGENE alone. ASCs over-expressing TRAIL using CPP inhibited growth of glioma U251MG cells both in vitro and in the U251MG xenograft model. Conclusion: CPP can be used as an enhancer for genetically manipulating ASCs and tumor treatment.