TY - JOUR T1 - Establishment of a Novel Temozolomide Resistant Subline of Glioblastoma Multiforme Cells and Comparative Transcriptome Analysis With Parental Cells JF - Anticancer Research JO - Anticancer Res SP - 2333 LP - 2347 DO - 10.21873/anticanres.15008 VL - 41 IS - 5 AU - HAI-ANH HA AU - JAI-SING YANG AU - FUU-JEN TSAI AU - CHANG-WEI LI AU - YIH-DIH CHENG AU - JIANYONG LI AU - MANN-JEN HOUR AU - YU-JEN CHIU Y1 - 2021/05/01 UR - http://ar.iiarjournals.org/content/41/5/2333.abstract N2 - Background/Aim: Glioblastoma multiforme (GBM) is a lethal disease with a high rate of chemoresistance to temozolomide (TMZ). The aim of the study was to establish a TMZ-resistant subline from the GBM-8401 cell line to determine the mechanisms of resistance and identify novel effective therapeutics for TMZ-resistant GBM. Materials and Methods: Comparative transcriptome analysis of GBM-8401/TMZR cells and the parental line was performed using Ion Torrent sequencing. Differentially expressed genes (DEGs) between the GBM-8401/TMZR and GBM-8401 cell lines were analyzed. Results: Transcriptomic profiling of GBM-8401/TMZR cells revealed DEGs involved in the retinoblastoma (RB) signaling, DNA damage response (DDR) pathway, and DNA repair mechanisms. Conclusion: In vitro and in vivo cell-based GBM models should be used in further biomedical studies to investigate the underlying mechanisms of TMZ-resistant GBM. ER -