RT Journal Article
SR Electronic
T1 IL-39 Reduces Proliferation and Promotes Apoptosis of Bladder Cancer by Altering the Activity of Cyclin E and Fas
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2239
OP 2245
DO 10.21873/anticanres.15000
VO 41
IS 5
A1 HUAPING XIAO
A1 HAMZA ALISIC
A1 BRANDON T. REIMAN
A1 ZULIANG DENG
A1 ZIWEN ZHU
A1 NATHAN T. GIVENS
A1 QIAN BAI
A1 ANDREW TAIT
A1 MARK R. WAKEFIELD
A1 YUJIANG FANG
YR 2021
UL http://ar.iiarjournals.org/content/41/5/2239.abstract
AB Background/Aim: This study was designed to investigate the effect of IL-39 on T24 bladder cancer (BC) cell line survival and growth. Materials and Methods: In order to assess the direct effect of IL-39 on survival, proliferation, and apoptosis of T24 BC cells, we utilized a clonogenic survival assay, a cell proliferation assay, and caspase-3 activity kits. Potential proliferative and apoptotic molecular mechanisms were evaluated by RT-PCR. Results: Treatment of T24 BC cells with IL-39 resulted in a significant reduction in the percentage of colonies. The anti-tumor effect of IL-39 on T24 bladder cancer cells correlated strongly with a decrease in cyclin E, in combination with an increase in the mRNA levels of Fas. Conclusion: IL-39 impedes the growth and survival of T24 BC cells by inhibiting growth and promoting apoptosis. This ability to modulate gene transcription in neoplastic cells shows promise and warrants further research in immunotherapy.