TY - JOUR T1 - IL-39 Reduces Proliferation and Promotes Apoptosis of Bladder Cancer by Altering the Activity of Cyclin E and Fas JF - Anticancer Research JO - Anticancer Res SP - 2239 LP - 2245 DO - 10.21873/anticanres.15000 VL - 41 IS - 5 AU - HUAPING XIAO AU - HAMZA ALISIC AU - BRANDON T. REIMAN AU - ZULIANG DENG AU - ZIWEN ZHU AU - NATHAN T. GIVENS AU - QIAN BAI AU - ANDREW TAIT AU - MARK R. WAKEFIELD AU - YUJIANG FANG Y1 - 2021/05/01 UR - http://ar.iiarjournals.org/content/41/5/2239.abstract N2 - Background/Aim: This study was designed to investigate the effect of IL-39 on T24 bladder cancer (BC) cell line survival and growth. Materials and Methods: In order to assess the direct effect of IL-39 on survival, proliferation, and apoptosis of T24 BC cells, we utilized a clonogenic survival assay, a cell proliferation assay, and caspase-3 activity kits. Potential proliferative and apoptotic molecular mechanisms were evaluated by RT-PCR. Results: Treatment of T24 BC cells with IL-39 resulted in a significant reduction in the percentage of colonies. The anti-tumor effect of IL-39 on T24 bladder cancer cells correlated strongly with a decrease in cyclin E, in combination with an increase in the mRNA levels of Fas. Conclusion: IL-39 impedes the growth and survival of T24 BC cells by inhibiting growth and promoting apoptosis. This ability to modulate gene transcription in neoplastic cells shows promise and warrants further research in immunotherapy. ER -