RT Journal Article
SR Electronic
T1 Significance of RNA Reference in Tumour-related Gene Expression Analyses by cDNA Array
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1397
OP 1407
VO 25
IS 2B
A1 LAYTRAGOON-LEWIN, NONGNIT
A1 LAGERLUND, MAGNUS
A1 LUNDGREN, JAN
A1 NORDLANDER, BRITT
A1 ELMBERGER, GÖRAN
A1 SÖDERGREN, TOWE
A1 LAGERROS, CHRISTOFER
A1 RUTQVIST, LARS ERIK
A1 LEWIN, FREDDI
YR 2005
UL http://ar.iiarjournals.org/content/25/2B/1397.abstract
AB The cDNA array technique is an efficient approach for studying the expression of a large number of genes in a single experiment. The cDNA array analysis indicates the relative level of corresponding gene expression from a specimen and a reference. Our investigation was performed to address the significance of reference RNA on the outcome of the cancer-related gene expression profile obtained from cDNA array analysis. Human head and neck squamous cell carcinoma (HNSCC) biopsies and 5 sources of RNA reference were used for this purpose. In these biopsies, each individual patient expressed a unique set of genes both in normal and tumour tissue. It is important to note that 5 striking patterns of tumour-related gene expression were obtained according to the 5 references used. Significant differences in 60%, 16%, 15% and 15% of the genes expressed were shown when autologous normal matched tissue biopsy references were compared to pooled cell lines, allogenic normal mixed cell types, tumours or allogenic normal matched cell type references, respectively. Thus, theoretically and our study suggested that patient autologous normal cells matching with the tumour type should be the most suitable reference in cDNA array for the identification of individual tumour gene profiles with clinical purpose. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved