TY - JOUR T1 - Expression of Retinoblastoma and p53 Pathway-related Proteins in Resectable Invasive Ductal Carcinoma of the Pancreas: Potential Cooperative Effects on Clinical Outcome JF - Anticancer Research JO - Anticancer Res SP - 1361 LP - 1368 VL - 25 IS - 2B AU - KOJI HASHIMOTO AU - YOSHINORI NIO AU - MAKOTO KOIKE AU - MASAYUKI ITAKURA AU - SEIJI YANO AU - TETSUYA HIGAMI AU - RIRUKE MARUYAMA Y1 - 2005/03/01 UR - http://ar.iiarjournals.org/content/25/2B/1361.abstract N2 - Background: The retinoblastoma protein (RB) is an important cell cycle regulator. RB also plays an important role in the regulation of apoptosis, which is mediated by interaction of p53 signaling mediators. The present study was designed to assess the clinicopathological significance of RB and p53 pathway-related proteins (p53, p21WAF1/CIP1 and Bax) expression in resectable invasive ductal carcinoma (IDC) of the pancreas. Materials and Methods: The present study included 79 pancreatic IDC patients, who received surgery between 1982 and 2002. The expression of RB and p53 pathway-related proteins (p53, p21WAF1/CIP1 and Bax) were analyzed by immunohistochemistry. Results: RB was expressed in 45 (57%) of the 79 patients. RB expression correlated significantly with histological grade and grade of nodal involvement. The positive rate of p53, p21WAF1/CIP1 and Bax expression was 49%, 48% and 67%, respectively. RB expression alone did not have a significant effect on patient survival. However, coexpression analysis of RB and p53 pathway-related proteins indicated that, in the patients with RB (+) IDC, the p21WAF1/CIP1 (+) group had a significantly higher survival rate than the p21WAF1/CIP1 (-) group. On the other hand, in the patients with RB (-) IDC, the Bax (+) group had a significantly higher survival rate than the Bax (-) group. Multivariate analysis indicated that, in the RB (-) group, pTNM stage, adjuvant chemotherapy and Bax expression were significant variables. Conclusion: The evaluation of RB expression combined with the mediators of the p53 pathway, p21WAF1/CIP1 and Bax, may provide more accurate information regarding clinical outcome, beyond that which is provided by RB expression alone. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -