@article {OGO1771, author = {AYAKO OGO and SACHI MIYAKE and HISAKO KUBOTA and MASAHARU HIGASHIDA and HIDEO MATSUMOTO and FUSAKO TERAMOTO and TOSHIHIRO HIRAI and TOMIO UENO}, title = {The Mechanism of the Synergistic Anticancer Effect of CDDP and EPA in the TE1 Cell Line}, volume = {41}, number = {4}, pages = {1771--1778}, year = {2021}, doi = {10.21873/anticanres.14942}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Eicosapentaenoic acid (EPA) is an unsaturated fatty acid with various bioactivities, including antitumor effects. We previously reported a synergistic antitumor effect of cisplatin (CDDP) and EPA. Here, we examined the underlying mechanism. Materials and Methods: The human oesophageal cancer cell line TE-1 was treated with the combination of EPA and CDDP. Nuclear translocation of NF-κB, a transcription factor involved in cytokine production, was detected by immunohistochemistry. IL-6 levels were measured by ELISA. Apoptosis and cell cycle distribution were evaluated by flow cytometry. Results: Nuclear translocation of NF-κB in TE-1 cells was synergistically decreased by CDDP and EPA. IL-6 production was increased following treatment with CDDP, but treatment with EPA decreased IL-6 levels. Apoptosis was synergistically induced by CDDP and EPA. A G2/M cell cycle arrest was observed with the combination of CDDP and 150 μM EPA, and S phase arrest with the combination of CDDP and 100 μM EPA. Conclusion: The combination of CDDP and EPA synergistically suppresses NF-κB nuclear translocation and increases apoptosis by inducing cell cycle arrest at the S or G2/M phase.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/41/4/1771}, eprint = {https://ar.iiarjournals.org/content/41/4/1771.full.pdf}, journal = {Anticancer Research} }