RT Journal Article
SR Electronic
T1 Micronucleus Formation in Primary Oropharyngeal Epithelial Cells Reveals Mutagenicity of Cement Dusts
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1849
OP 1858
DO 10.21873/anticanres.14951
VO 41
IS 4
A1 PAWLICZAK, JACQUELINE
A1 KOLB, MARLEN
A1 BAUER, MARIO
A1 GMINSKI, RICHARD
A1 DIETZ, ANDREAS
A1 WICHMANN, GUNNAR
YR 2021
UL http://ar.iiarjournals.org/content/41/4/1849.abstract
AB Background/Aim: Epidemiological studies showed an increased risk of developing laryngeal head and neck squamous cell carcinoma (HNSCC) for employees working in the construction business. This suggested a causal link between exposure to cement particles and development of HNSCC but data were missing. Materials and Methods: We established an Organisation for Economic Co-operation and Development (OECD) guideline 487-conform micronucleus assay (MNA) using oropharyngeal mucosa-derived primary epithelial cells (OPCs) ex vivo. OPCs from healthy mucosa of 52 donors were cultured in vitro and incubated with serial concentrations of two common cement particles. Mitomycin C was used as a soluble positive control, and TiO2 and DQ12 were used as negative and positive particle controls. Bi-nucleated cells were counted and the mitotic index (MI) was determined. Subsequently, micronuclei-containing bi-nucleated cells (MN+) were counted. Results: Cement particles, in concentrations not significantly reducing ex vivo proliferation according to mitotic index, dose-dependently increased micronuclei formation. Conclusion: Through the establishment of an OECD guideline 487 conform MNA, we demonstrate the mutagenic effects of cement on human OPCs.