RT Journal Article
SR Electronic
T1 Down-regulation of Glutathione Peroxidase 4 in Oral Cancer Inhibits Tumor Growth Through SREBP1 Signaling
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1785
OP 1792
DO 10.21873/anticanres.14944
VO 41
IS 4
A1 FUKUDA, MASAKATSU
A1 OGASAWARA, YUDAI
A1 HAYASHI, HIROYASU
A1 OKUYAMA, AYAKO
A1 SHIONO, JUNYA
A1 INOUE, KATSUYUKI
A1 SAKASHITA, HIDEAKI
YR 2021
UL http://ar.iiarjournals.org/content/41/4/1785.abstract
AB Background/Aim: This study aimed to elucidate the role of glutathione peroxidase 4 (GPX4) on the sterol regulatory element binding proteins (SREBPs)-proliferation pathway in oral cancer cells, and determine its protein expression in oral cancer tissues. Materials and Methods: Quantitative RT-PCR and immunoblot analysis were carried out. Cell viability assay, apoptosis detection assay, immunohistochemistry and GPX4 knockdown were performed. Results: The levels of both GPX4 mRNA and protein were highest in SAS cells. GPX4 knockdown in SAS cells, a human oral squamous cell carcinoma cell line, using GPX4 siRNA resulted in a reduction in cell number, which appeared to be due to non-apoptotic cell death such as ferroptosis. Furthermore, SREBP was clearly down-regulated by GPX4 knockdown in SAS cells. Immunopositivity for GPX4 was revealed on the membrane of human oral squamous cell carcinoma cells, and this was correlated with p53 immunoreactivity. Conclusion: GPX4 appears to play an important role in oral cancer proliferation.