PT  - JOURNAL ARTICLE
AU  - MASAKATSU FUKUDA
AU  - YUDAI OGASAWARA
AU  - HIROYASU HAYASHI
AU  - AYAKO OKUYAMA
AU  - JUNYA SHIONO
AU  - KATSUYUKI INOUE
AU  - HIDEAKI SAKASHITA
TI  - Down-regulation of Glutathione Peroxidase 4 in Oral Cancer Inhibits Tumor Growth Through SREBP1 Signaling
AID  - 10.21873/anticanres.14944
DP  - 2021 Apr 01
TA  - Anticancer Research
PG  - 1785--1792
VI  - 41
IP  - 4
4099  - http://ar.iiarjournals.org/content/41/4/1785.short
4100  - http://ar.iiarjournals.org/content/41/4/1785.full
SO  - Anticancer Res2021 Apr 01; 41
AB  - Background/Aim: This study aimed to elucidate the role of glutathione peroxidase 4 (GPX4) on the sterol regulatory element binding proteins (SREBPs)-proliferation pathway in oral cancer cells, and determine its protein expression in oral cancer tissues. Materials and Methods: Quantitative RT-PCR and immunoblot analysis were carried out. Cell viability assay, apoptosis detection assay, immunohistochemistry and GPX4 knockdown were performed. Results: The levels of both GPX4 mRNA and protein were highest in SAS cells. GPX4 knockdown in SAS cells, a human oral squamous cell carcinoma cell line, using GPX4 siRNA resulted in a reduction in cell number, which appeared to be due to non-apoptotic cell death such as ferroptosis. Furthermore, SREBP was clearly down-regulated by GPX4 knockdown in SAS cells. Immunopositivity for GPX4 was revealed on the membrane of human oral squamous cell carcinoma cells, and this was correlated with p53 immunoreactivity. Conclusion: GPX4 appears to play an important role in oral cancer proliferation.