@article {FUKUDA1785, author = {MASAKATSU FUKUDA and YUDAI OGASAWARA and HIROYASU HAYASHI and AYAKO OKUYAMA and JUNYA SHIONO and KATSUYUKI INOUE and HIDEAKI SAKASHITA}, title = {Down-regulation of Glutathione Peroxidase 4 in Oral Cancer Inhibits Tumor Growth Through SREBP1 Signaling}, volume = {41}, number = {4}, pages = {1785--1792}, year = {2021}, doi = {10.21873/anticanres.14944}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: This study aimed to elucidate the role of glutathione peroxidase 4 (GPX4) on the sterol regulatory element binding proteins (SREBPs)-proliferation pathway in oral cancer cells, and determine its protein expression in oral cancer tissues. Materials and Methods: Quantitative RT-PCR and immunoblot analysis were carried out. Cell viability assay, apoptosis detection assay, immunohistochemistry and GPX4 knockdown were performed. Results: The levels of both GPX4 mRNA and protein were highest in SAS cells. GPX4 knockdown in SAS cells, a human oral squamous cell carcinoma cell line, using GPX4 siRNA resulted in a reduction in cell number, which appeared to be due to non-apoptotic cell death such as ferroptosis. Furthermore, SREBP was clearly down-regulated by GPX4 knockdown in SAS cells. Immunopositivity for GPX4 was revealed on the membrane of human oral squamous cell carcinoma cells, and this was correlated with p53 immunoreactivity. Conclusion: GPX4 appears to play an important role in oral cancer proliferation.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/41/4/1785}, eprint = {https://ar.iiarjournals.org/content/41/4/1785.full.pdf}, journal = {Anticancer Research} }