RT Journal Article
SR Electronic
T1 Development of Antigen-specific Chimeric Antigen Receptor KHYG-1 Cells for Glioblastoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1811
OP 1819
DO 10.21873/anticanres.14947
VO 41
IS 4
A1 CHUNG HYO KANG
A1 YEONGRIN KIM
A1 SO MYOUNG LEE
A1 SANG UN CHOI
A1 CHI HOON PARK
YR 2021
UL http://ar.iiarjournals.org/content/41/4/1811.abstract
AB Background/Aim: Glioblastoma is the most common cancer among primary brain tumors, however, its prognosis and treatment advances are very poor. Here, we investigated whether c-Met, FOLR1, and AXL proteins are promising targets for chimeric antigen receptor (CAR) T-cell therapy, for they are known to be over-expressed in a variety of solid tumors. Materials and Methods: CAR constructs were prepared and CAR KHYG-1 cells targeting c-Met, FOLR1, or AXL were made by lentiviral transduction. The activity of CAR KHYG-1 cells against cancer cells was measured by cytokine secretion and cell lysis assays. Results: c-Met and AXL were over-expressed in most glioblastoma cell lines (11/13), but not in neuroblastoma cell lines (0/8). FOLR1 was over-expressed only in one among 16 glioblastoma cell lines. Our antigen-specific CAR KHYG-1 cells eradicated target positive glioblastoma cells selectively. Conclusion: Anti-c-Met and anti-AXL CAR NK or T cells could be effective in glioblastoma cells.